SYNCELL INC.

Microscoop, an innovative microscopy platform, is designed to fill the needs of identifying novel protein constituents at the protein-protein interaction level. Existing mass spectrometry-based unbiased methods often lack subcellular spatial precision or sensitivity for high-precision proteomic discovery, both for cell and tissue samples.  Our solution is "optoproteomics," a cutting-edge technique that combines microscopy, mechatronics, photochemistry, and advanced image processing (either deep learning or traditional methods) to enable ultrahigh-content in situ photo-biotinylation. The Microscoop system photolabels proteins at user-defined regions of interest (ROIs) under a microscope, utilizing directed photochemistry one field of view (FOV) at a time for tens of thousands of FOVs with similar morphological features. Here’s how it works: 1) Microscopy Integration: The system uses advanced microscopy to visualize and define ROIs within cell or tissue samples. 2) Directed Photochemistry: Utilizing precise optics and photochemistry, the system photolabels proteins within these ROIs at 240-nm resolution. 3) High-Throughput Capability: This process is repeated across tens of thousands of FOVs, overcoming protein amplification problem by protein accumulation. 4) Extraction and Analysis: Photolabeled proteins are then extracted and analyzed via mass spectrometry, revealing both known and novel protein players specific to the ROIs. Read more - https://lnkd.in/gMpAv3ax #Syncell #SyncellTechnology #Optoproteomics

Syncell is pleased to announce our attendance at the FASEB Molecular Metabolism: Cells and Systems conference. We will be showcasing our research poster titled "Identifying novel peri-droplet-mitochondrial interface proteins of oleic acid-treated HepG2 cells using microscopy-guided subcellular spatial protein purification." This presentation highlights our innovative approach and the capabilities of Microscoop technology in identifying previously unrecognized protein constituents at the mitochondria-LD interface, paving the way for further functional examination associated with lipid regulation and Non-alcoholic fatty liver disease pathogenesis. Connect with our scientist Yen Ming Lin. The conference covers a wide range of important topics, such as emerging methods for analyzing the compartmentalization of metabolism at the cell and tissue levels, the latest advances in regulating metabolic physiology and organ crosstalk, outstanding questions and current areas of research in targeting GLP1, and how metabolites can function as signaling molecules. We look forward to participating in insightful discussions and networking with fellow researchers at the FASEB Molecular Metabolism: Cells and Systems Conference. #Syncell #MolecularMetabolism #FASEB2024 #MMCSRC

Misfolded protein aggregates are a hallmark of neurodegenerative conditions like Alzheimer's, Parkinson's, ALS, and Huntington's disease, creating toxic environments that drive disease progression. For example, Alzheimer's disease is marked by β-amyloid plaques and tau tangles that disrupt brain function, while Parkinson's disease features α-synuclein aggregates known as Lewy bodies. Understanding these protein aggregates is crucial for developing effective treatments for these challenging conditions. The blog explores a new spatial proteomics discovery technique that enables researchers to pinpoint the exact location and composition of protein aggregates within diseased tissues. Read more - https://lnkd.in/gEjdyz6E

Syncell is set to attend FENS Forum 2024 in Vienna, Austria. Our team is eager to share, learn, and collaborate with the neuroscience community. We invite you to meet us at booth #403B to uncover new perspectives on the challenges facing our field. 📍 Booth #403B Hall B Ground Level #FENS2024 #Neuroscience #Syncell #ProteomicDiscovery

Syncell Microscoop™ platform is specifically designed to study subcellular structures, including the nucleus and nucleolus, with high precision through photolabeling at the submicron-scale. Using this technology, we harvested photolabeled nuclei for LC-MS/MS analysis, identifying 4,820 proteins with high confidence.   The specificity of the spatially labeled nuclear proteome was evaluated by determining the percentage of true positive proteins known as nuclear proteins among the obtained proteomes. Remarkably, out of the 1,316 proteins that showed differential enrichment, 1,207 were annotated as nuclear proteins, reflecting a 92% true positive rate compared to the existing nuclear protein database.   Read full article here: https://lnkd.in/gjKcUH_E #Syncell #Sensitivity #Specificity #SpatialProteomics

We are pleased to share Syncell has been invited to give a talk at the Spatial Omics VIB conference in Belgium this year. Our founder Jung-Chi Liao will be presenting "Microscopic Scooping for Subcellular Spatial Proteomics Discovery" on June 14 from 11:10-11:35am. If you are attending the event, swing by to check out our poster and booth. Spatial technologies allow researchers to accurately map out the role of each cell in an organ, making it possible to identify specific biological scenarios and anticipate future developments in cellular environments. We look forward to contributing to the discussion and advancing our understanding of spatial proteomics. #SpatialOmics #SpatialProteomics #VIBConference #InvitedTalk

Syncell is pleased to share that we will attend the GRC Single-Cell Cancer Biology conference. During the conference, Syncell will be showcasing our research poster titled "Identification of the novel immune synapse-localized proteome for immuno-oncology using Microscoop-induced targeted photo-biotinylation." This presentation highlights our innovative approach and the capabilities of Microscoop technology in identifying immune synapse-localized proteins, contributing to advancements in immuno-oncology research. This conference covers a wide range of important topics such as pre-malignancies and clonal evolution, intra-tumor heterogeneity, cell-cell interactions in the tumor microenvironment, technical advances in single-cell protocols and computational methods for data analysis and modeling, single-cell technologies in clinical practice, liquid biopsy, and single-cell multi-omics. We look forward to participating in insightful discussions and networking with fellow researchers at the GRC Single-Cell Cancer Biology conference. #Syncell #SingleCellCancer #ImmunoOncology #GRC2024

Discover the power of precision with Microscoop™ – the forefront of image-guided proteomics! 🎯Our platform is redefining protein isolation by zeroing in on specific regions within cells and tissue samples. No guesswork here – just robust, hypothesis-free protein identification powered by microscopic analysis. 🔬 With Microscoop™, experience the power of precise photo-biotinylation utilizing ultrafast femtosecond pulses for two-photon illumination, pinpointing targets at the scale of individual organelles. This technology opens doors to a detailed exploration of subcellular protein dynamics and relationships, providing critical insights into the intricate subcellular structures that drive biological discovery. Learn more about the broad application of Microscoop in our new #AppNote - https://lnkd.in/gtz6_q6B #Microscoop #Proteomics #ProteinDiscovery #LaserNanoPulldown #ScoopBiology

Syncell is attending the FASEB-hosted Nuclear Bodies: Hubs of Genomic Activity Research Conference in New York, running until June 7th. Our principal scientist, Mei Zhang, will present on June 5th at 8:55 pm, discussing “Hypothesis-Free Discovery of the Subcellular Proteome: A Total-Sync Ultra-Content Microscopic Opto-Biotinylation System.” This conference gathers international experts to delve into the molecular biology, cell biology, and biophysics of nuclear structures. These nuclear bodies, distinct from chromosomes, interact with the genome and play roles in various pathological states, with a special focus on cancer this year. Spatial proteomic discovery is vital for understanding nuclear bodies, as it helps reveal novel protein interactions and their implications in disease. #FASEB #NBGSRC #SpatialProteomics

Traditionally, spatial biology research on tissue biospecimens relies on co-staining with multiple known markers to map protein distribution through imaging. Microscoop™ revolutionizes this process by enabling hypothesis-free identification of proteomes within submicron structures in tissue biospecimens, whether formalin-fixed, paraffin-embedded (FFPE), or fresh frozen. Using only one preliminary marker to indicate the protein of interest (POI), Microscoop™, in conjunction with LC-MS/MS, facilitates targeted biotin-tagging and identification of the proteome near the POI, enriching researchers with biologically significant insights. In this application note, we showcase Microscoop's versatile applications in brain, intestine, and lung tissues. Link - https://lnkd.in/gxrcN_53