All Questions
Tagged with optics microscopy
145
questions
1
vote
0
answers
15
views
Can we use prism in fluorescence microscope instead of dichroic mirror?
For the light path outlined here, if we use a prism instead of a dichroic mirror, the excitation light can also pass through the prism, but the emission filter can block the excitation wavelength if ...
- 11
0
votes
2
answers
56
views
Why is the shorter the wavelength, the smaller the object you can image? and vice-versa?
I know that the shorter the wavelength the smaller the object you can image clearly. Why wavelength matters in imaging something? How having big wavelength wont let u image smaller object, like if u ...
1
vote
2
answers
53
views
When to add signals coherently and incoherently?
I just learned in transmission electron microscopy about coherent and incoherent summation. I can't seem to wrap my head around the fact that intensities can be added incoherently. Why can you do that?...
- 13
0
votes
1
answer
34
views
Limiting factors in traditional microscopy
I have been researching Entanglement-Enhanced Microscopy, and the overall breakthrough seems to be obtaining a resolution of $1/N$ as opposed to $1/\sqrt N$. This led me to ask what the limiting ...
0
votes
0
answers
16
views
Where is the final image located at in a compound microscope?
In a compound microscope, is there a standard as to where the final image should be located? After doing some research, I arrived at either the final image should be located at infinity (to be focused ...
- 186
0
votes
0
answers
23
views
Simple interference confocal microscope
I have the two articles:
The first one: First article
and
The second one: Second article
After reading the first article I understood while reading that using the detector A we get confocal ...
2
votes
2
answers
93
views
Why does Near Infrared (NIR) light allow deeper imaging in biological tissue in Multiphoton Microscopy?
I have been reading this paper on multiphoton microscopy (https://www.nature.com/articles/nphoton.an.2010.2) and I am very confused about something. Here is a quote from the paper:
"The NIR ...
- 21
0
votes
0
answers
31
views
Why transparent object altering only the phase of light but not the frequency or amplitude? Is there exception?
I have learned the fascinating phase contrast microscope from Eugene's textbook optics that made transparent object become visible.
From page 647, he said that
In contradistinction, it is often ...
- 21
3
votes
1
answer
63
views
Can a system of lenses or compound lens have a higher numerical aperture than a single element in the system?
I am attempting to create a simple Abbe condenser for a microscope, which typically consists of two converging lenses.
Ideally, I would like to achieve a numerical aperture for the condenser of ...
- 33
1
vote
1
answer
60
views
Is reflection from pockel cell normal?
We are trying to align a laser for a 2-photon microscope and we noticed a reflection from the pockel cell. The reflection can be caught on the edge of the waveplate or the iris that are before the ...
0
votes
3
answers
154
views
Imaging and illumination plane in microscope
I've been delving into the intricacies of achieving Kohler illumination in microscopy, and the term 'illumination and image-forming rays' consistently surfaces. Most resources emphasize the ...
3
votes
0
answers
99
views
Out-of-focus color in reflective microscopy with Köhler EPI-illumination
It is well known that Köhler illuminator in optical microscopy is designed in a way to ensure uniform illumination of the field, often with adjustable apertures to allow as little light as possible to ...
- 2,424
1
vote
0
answers
44
views
Can the principle of helium ion microscopes be generalized to heavier noble gasses for suitable substances?
I have heard helium ion microscopes offer extremely high resolution of features due to the shorter debroglie wavelength of the helium atoms.
Naturally I was curious if anyone has built something using ...
- 2,989
0
votes
0
answers
39
views
Beam diameter of a fluoresence emitter after an objective
I try to calculate the beam diameter after a high NA objective that focuses on a point-like emitter that emits fluoresence light.
To my basic understanding, I get this by computing the Entrance pupil (...
- 131
0
votes
0
answers
44
views
Why would an object disappear when switching from monocular to binocular vision?
I have just used a compound binocular microscope which has an ‘eyepiece graticule’ (ruler in arbitrary units) in the right eyepiece lens.
If you close your left eye (or occlude the left lens), the ...
