May Parker is the aunt of the late Peter Parker. Following the death of her nephew, she discovered that variants of Peter were starting to appear in her universe and she began granting refuge to three of them - Spider-Man Noir, SP//dr, and Spider-Ham - to form the Spider-Gang, providing them access to her nephew's base of operations and equipment.

Later, she was visited by three more versions of Spider-Man, including Miles Morales and Gwen Stacy, and became emotional upon seeing that an alternate version of Peter, who had conversely lost his own Aunt May, was among them. Her house was later attacked by Kingpin and his lackeys. She was lastly seen when Miles returned to her house, helping him design his new black-and-red suit and gifting him web-shooters that she designed herself.^[1]

May would eventually retire to Florida, saying goodbye to Miles as she did.^[2]

• May Parker is voiced by Lily Tomlin in Spider-Man: Into the Spider-Verse and Spider-Man: Across the Spider-Verse.

• It appears she and Doctor Octopus know each other, since they're on first name basis. She even called her "Liv," a nickname only her friends called her.^[1]