Earth-616B is the home reality of Peter B. Parker. He was bitten by a radioactive spider in his teens and, after the death of his beloved uncle Ben, became the sole protector of New York City known as Spider-Man. After a two-decade career as the city's hero, Peter's life turned out for the worse. His aunt May died, and Peter divorced his wife Mary Jane Watson, spiraling him into a depressive state.

Peter was plucked out of his reality following a Super Collider explosion in the parallel reality of Earth-1610B, whose Peter Parker lived a perfect life until his untimely death at the hands of the Kingpin. Peter B. encountered Miles Morales, a young boy who had been similarly bitten by a radioactive spider, and went on to become his mentor in an adventure that resulted in Miles and Peter B. teaming up with other displaced spider-people to stop the Kingpin from rebuilding the Super Collider. Inspired by Miles, Peter made amends with Mary Jane once he returned to his home reality, and fathered a daughter named May.^[1][3]

• Prior to being given an official reality number, this universe was referred as Earth-TRN701 as a part of our Temporary Reality Numbers classification system.

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• This reality is designated "E-616" in Spider-Man: Into the Spider-Verse and "Earth-616" in Spider-Man: Across the Spider-Verse - The Art of the Movie and in the Official Film Screenplay. However, it cannot be the same Earth-616 as the Prime Marvel Universe comics due to many irreconcilable differences in their history. In Spider-Man: Across the Spider-Verse, Hobie Brown's jerry-rigged Dimensional Travel Watch designated the home reality of Peter B. Parker as "Earth-616B."

• A deleted version of Peter B. Parker's flashback sequence in Spider-Man: Into the Spider-Verse includes an appearance from the Hulk.