All Questions
Tagged with extraction chromatography
4
questions
3
votes
2
answers
121
views
Can I expect the same (or very close) peak areas from herbal extracts prepared with the same plant/solvent ratio?
I prepared an extract by using 100 mg of plant and 2 mL of ethanol, filtered and injected 20 μl into the HPLC system equipped with Waters® XSelect (150 mm × 4.6 mm, 5 μm) column. If I prepare an ...
- 43
2
votes
1
answer
79
views
How can over grinding lead to reduced API extraction in HPLC analysis?
I am looking for some advice regarding particle size distribution (PSD) & how this can be affected by grinding a tablet.
During an assay by HPLC, the method requires grinding of a tablet before ...
- 21
4
votes
2
answers
281
views
Why n-alkanes can be found in organic solvent extract of rubber?
Sorry for the confusing title, I didn't know how to describe this better. So, if we douse rubber, say, a bicycle tire or a certain type of rubber glove, with organic solvent (n-heptane), we can find n-...
- 121
2
votes
0
answers
206
views
Process Chromatography vs Solid Phase Extraction techniques
Process Chromatography is typically performed by flowing eluent from the top to the bottom of the column. The standard method of solid phase extraction at industrial scale e.g. to extract natural ...
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