Questions tagged [enzyme-kinetics]
Questions related to enzyme kinetics.
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How can a Michaelis–Menten formalism be used when enzyme concentration isn't constant?
I understand that $V_\mathrm{max} = k_3[\ce{E}]_0$ in ordinary Michaelis–Menten (MM) kinetics. According to the lecture notes provided by my university (I don't believe they are available online), ...
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Can glycerol slow down the kinetics of an inhibitor going to a binding pocket of a target enzyme?
I am currently doing enzymatic inhibition assays and in my current setup I observe that a commercial inhibitor is showing inhibition as expected. Still, the inhibition is not as strong as the ...
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Calculate IC50 from fluorescence kinetic
I have an assay in which a fluorescence signal is generated when the enzyme reaction progresses. The fluorescence signal increases until a maximum is reached. Than it keeps constant. The first 1 h ...
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Derivation of general binding equation (macromolecule with multiple binding sites)
So the last days I'm stuck at trying to solve the an equation - but I just can't (see the screenshot below.
So the idea is that you have a receptor or enzyme with multiple binding sites for a Ligand (...
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Enzymatic mechanism of Lipase
When lipase catalyses the hydrolysis of a triglyceride (oil/fat) which bond does it break and why?
What about in the case of saponification (triglyceride + NaOH) in the presence of lipase?
Is the same ...
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