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    $\begingroup$ What is the ratio of ethanol(probe) and water(acidic Mg solution)? Why not to buffer the water solution? pKa and pH of buffers are implied for water, not other solvents. $\endgroup$
    – Poutnik
    Commented Jun 10 at 10:00
  • $\begingroup$ @Poutnik: I agreed with you. fluorescent probe works in very dilute conditions so we usually dissolve them in suitable organic solvents in minimum volume and dilute it to required concentration using water (in here, using suitable buffer solution). $\endgroup$ Commented Jun 10 at 17:09