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$\begingroup$ Would the issues with the pH probes have made the results skewed enough to go completely in reverse from what was expected? Because we tested three trials for each temperatures and they all matched up, and the overall data made what would have been the correct trend except going in the opposite direction $\endgroup$– Renae ThompsonCommented Dec 9, 2022 at 20:10
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$\begingroup$ High temperature does reduce pH because Kw of water is a function of temperature. You have to do controls as well. Measure pH of pure water exactly the same way. $\endgroup$– ACRCommented Dec 9, 2022 at 21:20
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$\begingroup$ If the data are reproducible, you did measure something; the question is What? The easy possibilities are that the system is not equilibrated, reversing the order might detect that, the closed tube is bothersome. Try to find a way to work at constant pressure. The prepared 4 and 7 buffers have the temperature pH on the bottle; calibrate at the temperature and do not use the ATC probe. Record the millivolt values to ensure the probe is responding properly. Use a combo electrode with a flowing junction not a gel filled. you disagreed with the lit. find out why! $\endgroup$– jimchmstCommented Dec 10, 2022 at 4:47
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