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  • $\begingroup$ Thank you for your answer. I still have a few questions: So if I go with option C and compare absorption at a specific wavelength, I won’t need to utilize extinction coefficients, as that is only needed for Beer’s law, is that correct? Also, could you clarify why the other options would not be a good idea? I was thinking that I could still try comparing the data using one of the other options like b) where I could compare absorptions levels at each respective lambda max in addition to option C, but would that be redundant? $\endgroup$
    – Chem Student
    Commented Aug 13, 2021 at 16:11
  • 1
    $\begingroup$ You don't know why lambda max shifts. It could be a different composition of the absorbing species, solvent or pH effects (you could minimize those by measuring at the same pH even when incubating at different pH values), or something else. The community could help you further if you posted examples of two spectra with different maxima. In the end, you have to acknowledge that the task is complex, and that a single claimed concentration of "chlorophyll" will not do it justice. Nevertheless, it is possible to learn something from the data you collect, and make more general claims based on it. $\endgroup$
    – Karsten ♦
    Commented Aug 13, 2021 at 17:34