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1$\begingroup$ Same as with other molecules, you adjust the pH of the mobile phase to get the anion or cation depending on what you are detecting. At neutral pH zwitter ions carry no net charge but are polar species so do not interact with the column like other neutral molecules. $\endgroup$– WaylanderCommented Feb 13, 2020 at 11:13
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$\begingroup$ That is not the right structure for aspartate. You've drawn the side chain with an aldehyde group instead of a carboxylic acid. Also, the amino acid on the left, 2,4-diaminobutyric acid, is a bit unusual. It's perfectly similar to the "usual" proteinogenic amino acids, and the same methods would work OK with it, but you won't see it in most published methods. $\endgroup$– Curt F.Commented Feb 26, 2020 at 0:06
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