All Questions
Tagged with assembly sequence-analysis
5
questions
2
votes
2
answers
68
views
Longstitch error make: command: Command not found *** No rule to make target
I installed Longstitch and ran the test script with no issues. The output files matched the expected output files. But when I am now trying to run Longstitch on my own data I am getting this error.
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- 21
2
votes
1
answer
126
views
need bam file for pilon
I just ran an assembly on yeast genomes using Flye and I want to polish those assemblies with Pilon but it requires a sorted BAM file.
How do I make a BAM file of the resulting assembled.fasta?
- 1,033
0
votes
1
answer
74
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How to improve a genome assembly using Dovetail and PacBio assembly?
I have more of a conceptual question. I have two genome assemblies from the same plant, one from Dovetail technology (~998 Gb) and another is PacBio HiFi assembly (~1.1 Gb). The Dovetail assembly is ...
- 75
0
votes
2
answers
332
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How to know if the DNA sequence has been assembled and why is it important to know how it was assembled?
I have downloaded my FASTA format files, that have the DNA sequences of the coding region of the genes and the DNA sequence of the complete genome, from NCBI. How can I recognize if these sequences ...
1
vote
1
answer
188
views
Interpreting contig alignments to a reference genome
I have applied two de novo genome assembly tools to data from the run SRR12707453, corresponding to a phage (I downloaded the data and have no relation with the authors of the study).
Using rnaSPAdes ...
- 359