β-hydroxy-β-methylbutyrate (HMB) attenuates muscle and body weight loss in experimental cancer cachexia
- PMID: 21184031
- DOI: 10.3892/ijo.2010.885
β-hydroxy-β-methylbutyrate (HMB) attenuates muscle and body weight loss in experimental cancer cachexia
Abstract
β-hydroxy-β-methylbutyrate (HMB), a leucine metabolite, improves muscle mass and function. This study aimed at evaluating the effects of HMB administration in an experimental in vivo model of cancer cachexia (CC). Wistar rats were randomized to receive standard or 4% HMB-enriched chow. Rats from both groups were randomized to receive an i.p. inoculum of AH-130 cells (TB). All rats were weighed and sacrificed at day 24. Liver, heart and muscles were dissected and weighed. The protein levels of p-p70S6k, p-eIf2α, p-mTOR and p-4-EB-P1 were evaluated by Western blotting on gastrocnemius muscle (GSN). As expected, the growth of the AH-130 ascites hepatoma induced significant carcass weight and GSN muscle loss. HMB treatment significantly increased GSN and heart weight in controls (p=0.002 and p<0.001, respectively). In HMB-treated TB, body weight was not lost but significantly (p=0.003) increased, and GSN loss was significantly (p=0.04) attenuated with respect to TB. Phosphorylated eIF2α markedly decreased in TB-rats vs. C. Feeding the HMB-enriched diet resulted in decreased p-eIF2α levels in control animals, while no changes could be observed in the TB group. Phosphorylated p70S6K and phosphorylated mTOR were markedly increased by HMB treatment in controls and further increased in TB. Phosphorylated 4-EB-P1 was markedly increased in TB but substantially unaffected by HMB treatment. Administration of HMB attenuates body weight and muscle loss in experimental CC. Increased phosphorylation of key anabolic molecules suggests that these actions are mediated by improved protein anabolism in muscle.
Similar articles
-
Attenuation of proteasome-induced proteolysis in skeletal muscle by {beta}-hydroxy-{beta}-methylbutyrate in cancer-induced muscle loss.Cancer Res. 2005 Jan 1;65(1):277-83. Cancer Res. 2005. PMID: 15665304
-
β-Hydroxy-β-methylbutyrate (HMB) enhances the proliferation of satellite cells in fast muscles of aged rats during recovery from disuse atrophy.Exp Gerontol. 2013 Sep;48(9):973-84. doi: 10.1016/j.exger.2013.06.005. Epub 2013 Jul 4. Exp Gerontol. 2013. PMID: 23832076
-
Signaling pathways initiated by beta-hydroxy-beta-methylbutyrate to attenuate the depression of protein synthesis in skeletal muscle in response to cachectic stimuli.Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E923-31. doi: 10.1152/ajpendo.00314.2007. Epub 2007 Jul 3. Am J Physiol Endocrinol Metab. 2007. PMID: 17609254
-
β-Hydroxy-β-methylbutyrate as a countermeasure for cancer cachexia: a cellular and molecular rationale.Anticancer Agents Med Chem. 2013 Oct;13(8):1188-96. doi: 10.2174/18715206113139990321. Anticancer Agents Med Chem. 2013. PMID: 23919746 Review.
-
Beta-Hydroxy-Beta-Methyl Butyrate (HMB): From Experimental Data to Clinical Evidence in Sarcopenia.Curr Protein Pept Sci. 2018;19(7):668-672. doi: 10.2174/1389203718666170529105026. Curr Protein Pept Sci. 2018. PMID: 28554316 Review.
Cited by
-
Influence of Amino Acids and Exercise on Muscle Protein Turnover, Particularly in Cancer Cachexia.Cancers (Basel). 2024 May 18;16(10):1921. doi: 10.3390/cancers16101921. Cancers (Basel). 2024. PMID: 38791998 Free PMC article. Review.
-
Effects of a multilevel intervention of resistance training with or without beta-hydroxy-beta-methylbutyrate in medical ICU patients during entire hospitalisation: a four-arm multicentre randomised controlled trial.Crit Care. 2023 Dec 15;27(1):493. doi: 10.1186/s13054-023-04698-x. Crit Care. 2023. PMID: 38102705 Free PMC article. Clinical Trial.
-
Does β-Hydroxy-β-Methylbutyrate Have Any Potential to Support the Treatment of Duchenne Muscular Dystrophy in Humans and Animals?Biomedicines. 2023 Aug 21;11(8):2329. doi: 10.3390/biomedicines11082329. Biomedicines. 2023. PMID: 37626825 Free PMC article. Review.
-
A Review of Nutraceuticals in Cancer Cachexia.Cancers (Basel). 2023 Jul 30;15(15):3884. doi: 10.3390/cancers15153884. Cancers (Basel). 2023. PMID: 37568700 Free PMC article. Review.
-
A Randomized, Double-Blind, Controlled Trial Assessing If Medium-Chain Triglycerides in Combination with Moderate-Intensity Exercise Increase Muscle Strength in Healthy Middle-Aged and Older Adults.Nutrients. 2023 Jul 24;15(14):3275. doi: 10.3390/nu15143275. Nutrients. 2023. PMID: 37513691 Free PMC article. Clinical Trial.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous